The qiaprep spin miniprep kit is designed for quick and convenient processing of 124 samples simultaneously in less than 30 minutes. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. Nov 30, 2005 the qiagen plasmid purification kits are designed to isolate and purify plasmid dna with specific consideration given to size and copy number. Qiagen qiaprep spin miniprep kit 27104 or 27106 with a. Here, we videorecorded the general procedures of miniprep through the qiagens qiaprep 8 miniprep kit, aiming to introducing this highly efficient technique to the. Qiagen guarantees the performance of all products in the manner described in our product literature.
The qiaprep miniprep system provides a fast, simple, and costeffective plasmid miniprep method for routine molecular biology laboratory applications. Qiaprep miniprep handbook washington state university. These are available online in convenient and compact pdf format. Each amplification curve represents an average of three independent dna free rna the quickrna miniprep plus kit is universal and accommodates any sample input including cells, tissue, blood etc. For technical assistance, please call tollfree 0080022446000, or find regional. Qiaprep miniprep kits are developed, designed, and sold for research purposes only. The procedure combines a unique buffer system with zymospin column technology to yield high quality total rna including small rnas 17200 nt in about 10 minutes. Qiaprep miniprep kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Quickstartprotocol qiaprep spin miniprep kit october 2010.
Qiagen tips can be left unattended, since the flow of buffer will stop when the meniscus reaches the upper frit in the column. Four different cell lines cos7, hek293, hela, and huh7 were grown to 8090% confluence and transfected with 100 ng of each plasmid, in complex with 0. Qiaprep spin miniprep kit highyield protocol english pdf. Australia qiagen pty ltd po box 25 clifton hill victoria 3068 abn 75 072 382 944 orders 0394893666 fax 0394893888 technical 0394893666 canada qiagen inc. Compare qiaprep spin miniprep kit from qiagen sample to insight on catalog number. Genelute hp plasmid miniprep kit 70 genelute plasmid miniprep kit 70. The resin bed will retain some buffer, and will not readily dry out, of the tips can be left unattended. This protocol is designed for purification of total dna from grampositive bacteria.
Please do not hesitate to contact us if you have any questions. Qiagen supports several covid19 testing protocols with test components, automation and a multiplex syndromic solution. Qiaprep miniprep handbook 062015 5 storage qiaprep miniprep kits should be stored dry at room temperature 1525c. Qiaprep miniprep handbook 112005 7 storage qiaprep miniprep kits should be stored dry at room temperature 1525c. Spin miniprep kit for purification of highquality plasmid dna. Cross reference of genelute to qiagen nucleic acid.
Equilibrate a qiagentip 20 by applying 1 ml buffer qbt, and allow the column to empty completely by gravity flow. Centrifuge for 15 seconds to elute the plasmid dna. Following is the miniprepqiagen kit protocol in biocoder, a highlevel programming language for expressing biology protocols. The provided dnarna shield stabilizes samples, allowing them to be stored without the need f. Following is the miniprepkit free high throughput protocol in biocoder, a highlevel programming language for expressing biology protocols. Equilibrate a qiagen tip 20 by applying 1 ml buffer qbt, and allow the column to empty completely by gravity flow.
Solutions that contain ethanol, isopropanol or mops should be sterilized by filtration only. Qiaprep spin miniprep kit protocol using 5ml collection tubes the qiaprep spin miniprep procedure can be performed using 5ml centrifuge tubes e. The qiagen plasmid purification kits are designed to isolate and purify plasmid dna with specific consideration given to size and copy number. Should any product fail to perform satisfactorily due to any reason other than misuse, qiagen will replace it free of charge or refund the purchase price. The kit utilizes silicabased membrane technology in the form of convenient spin columns. Qiaprep miniprep handbook 052012 5 storage qiaprep miniprep kits should be stored dry at room temperature 1525c. The procedure combines a unique buffer system with zymospin column technology to yield hig. What you see here is the autogenerated text ouput of the protocol that was coded up in biocoder see source code. Qiagen robotic systems are not available in all countries, please inquire. The genejet endofree plasmid maxiprep kit is designed for large scale isolation of high quality endotoxinfree plasmid dna from recombinant e. Purifying plasmid dna using the qiagen miniprep kit protocol. Qiagentips can be left unattended, since the flow of buffer will stop when the meniscus reaches the upper frit in the column. The quickrna miniprep plus kit is one of the most innovative rna isolation kits available, designed for the easy, reliable, and rapid isolation of dnafree rna from cells, all tissue types, whole blood, and biological fluids.
These are available online in convenient and compact pdf format at. Kits can be stored for up to 12 months without showing any reduction in performance and quality. C plasmid preparations with the genelute kit, comparing either the standard miniprep procedure or the miraprep using 1x volume of ethanol. Product description pkg size na0150 genelute hp plasmid miniprep kit 70 preps na0200s genelute hp plasmid midiprep kit 4 preps.
For a limited time, free trials of our new rna cleanup kits are also available. Genespin miniprep plasmid purification kit v2 for purification of highquality plasmid dna ver. The protocol describes the preliminary harvesting of bacteria and incubation with lysozyme to lyse their cell walls before dna purification. For more information, please refer to the qiaprep miniprep handbook, december. Qiagen will replace it free of charge or refund the purchase price. Timerun, 20g yield, ideal for fluorescent and radioactive sequencing, ligation, cloning. This kit is extremely easy to use, fast with uncomplicated steps, yields highly reproducible results and plasmid dna of both high quality and quantity. Under these conditions, if no expiration date is mentioned on the kit label, qiaprep miniprep kits can be stored for up to months without showing any reduction in performance and quality. Following is the miniprepkitfree high throughput protocol in biocoder, a highlevel programming language for expressing biology protocols. Although the development of plasmid isolation kits with silica spin columns reduced time and effort spent on plasmid purifications, high yields of plasmid dna still require significant time and labor table 1. Jul 20, 2015 this tutorial explains how to carry out a miniprep using the qiaprep spin miniprep kit provided by qiagen. Cap the microcentrifuge tube, and store eluted plasmid dna at 20c. The nucleospin plasmid miniprep kit is designed for the rapid 25 minutes, highyield up to 45 g, smallscale preparation of highpurity plasmid dna. The standard protocol on pages 1819 should be followed with the following modifications.
The quickrna miniprep kit is one of the most innovative rna isolation kits available, designed for the easy, reliable, and rapid isolation of dnafree rna from a wide range of cell up to 10 7 and tissue samples up to 50 mg. The quickrna miniprep plus kit is one of the most innovative rna isolation kits available, designed for the easy, reliable, and rapid isolation of dna free rna from cells, all tissue types, whole blood, and biological fluids. Total rna was isolated from 106 human epithelial cells with incolumn dnase treatment for both kits. We reserve the right to change, alter, or modify any product to enhance its performance. More information about biocoder can be found on my home page. Plasmid extraction from bacteria miniprep purification o f p lasmid d na from 1 5 m l e. Samples were analyzed on a bioanalyzer pico chip, with rin values and total yields shown below the lanes a. Plasmid purification 3 the boiling method is suitable for checking, if plasmid in e. Qiagen qiaprep spin miniprep kit, 50 preps, 1 to 100ml culture volume, 50l elution volume, molecular biology grade plasmid dna purification, silica technology, spin column format, manual processing, 30 min. Qiagen distributors please see the last page for contact information for your local qiagen distributor. The quickrna miniprep kit is an innovative product designed for the easy, reliable, and rapid isolation of dnafree rna from a wide range of cell up to 10 7 and tissue samples up to 50 mg. Kits can be stored for up to 12 months without showing any reduction in performance and qual ity.
Na0400s genelute hp endotoxin free plasmid maxiprep kit 4 preps na0400 genelute hp endotoxin free plasmid maxiprep kit 10 preps na0410 genelute hp endotoxin free plasmid maxiprep kit 25 preps related products catalog no. Qiaprep miniprep handbook 052004 7 storage qiaprep miniprep kits should be stored dry at room temperature 1525c. This is the protocol for qiagens qiaprep spin miniprep kit catalog numbers 27104 or 27106 with their newer version 2. Plasmid dna purification using the qiaprep spin miniprep kit and a. The qiaprep spin miniprep kit can be fully automated on the qiacube connect.
The kit is based on specific binding of plasmid dna to a silicagel membrane in high salt buffer followed by use of. The purified dna is suitable for transfection, cloning, sequencing, pcr, transformation, and. The quickrna miniprep kit is one of the most innovative rna isolation kits available, designed for the easy, reliable, and rapid isolation of dna free rna from a wide range of cell up to 107 and tissue samples up to 50 mg. These miniprep columns contain a unique silica membrane that binds up to 50 g of plasmid dna. See here or here for the handbook for the qiagen spin miniprep kit.
We continue to work tirelessly to support healthcare workers and patients in the fight against this pandemic. Plasmid dna encoding constitutively expressed gfp pegfpc2 was prepared using either monarch plasmid miniprep kit or qiagen qiaprep spin miniprep kit. The monarch plasmid miniprep kit is a rapid and reliable method for the purification of high quality plasmid dna. Qiagen, qiafilter, hispeed, and endofree plasmid kits, provide optimal transfection. The purchaser must determine the suitability of the product for its particular use. Apr 11, 2020 qiagen robotic systems are not available in all countries, please inquire. Formulas for qiagen kit buffers for long term storage, all buffers should be sterilized by filtration or autoclaving. Product description pkg size na0400s genelute hp endotoxinfree plasmid maxiprep kit 4 preps na0400 genelute hp endotoxinfree plasmid maxiprep kit 10 preps na0410 genelute hp endotoxinfree plasmid maxiprep kit 25 preps related products catalog no. Quickstart protocol february 2015 qiaprep spin miniprep kit the qiaprep spin miniprep kit cat. May 07, 2009 i have used the qiaprep miniprep kit to isolate plasmid dna for the last five years and feel that it is one of the best kits currently on the market. Each amplification curve represents an average of three independent dnafree rna the quickrna miniprep plus kit is universal and accommodates any sample input including cells, tissue, blood etc. Miniprepkitfree highthroughput protocol openwetware. The kit is based on specific binding of plasmid dna to a silicagel membrane in high salt buffer followed by use of a low salt buffer or water to facilitate elution.
Product description pkg size na0150 genelute hp plasmid miniprep kit 70 preps. Apply the supernatant from centrifugation to the qiagen tip 20 and allow it to enter the resin by gravity flow. Qiaprep spin miniprep kit 250 250 qiaprep spin columns. Formulas for qiagen kit buffers genesee scientific. The quickrna miniprep kit is an innovative product designed for the easy, reliable, and rapid isolation of dna free rna from a wide range of cell up to 10 7 and tissue samples up to 50 mg. In accordance with qiagens isocertified quality management system, each lot of the rneasy mini kit, rneasy protect mini kit, and rneasy plant mini kit is tested against predetermined specifications to ensure consistent product quality. Qiaprep miniprep kits use silicagelmembrane technology to eliminate the cumbersome steps associated with loose resins or slurries. I have used the qiaprep miniprep kit to isolate plasmid dna for the last five years and feel that it is one of the best kits currently on the market. Overnight bacterial culture with chassis containing plasmid of choice 1 5 ml plasmid purification kit i. They can be stored for at least 2 years without showing any reduction in performance, capacity, or quality of separation. This kit is extremely easy to use, fast with uncomplicated steps, yields highly reproducible results and plasmid dna of. Qiaprep spin miniprep kit from qiagen biocompare product. The provided dnarna shield stabilizes samples, allowing them to be stored without the need for immediate freezing or processing for up to one month.
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